Panel: “Protecting Our National Parks: What Should Be Done,” and William J. Lockhart, Outline: Problems and Issues that Must Be Addressed; and Some Preliminary Proposals for Solutions

26 pages

Panel: “Protecting Our National Parks: What Should Be Done,” and William J. Lockhart, Outline: Problems and Issues that Must Be Addressed; and Some Preliminary Proposals for Solutions

26 pages

BLM Land Planning and Consistency Obligations to Provide for Protection of Natural Values on Adjacent Protected Lands

60 pages

BLM Land Planning and Consistency Obligations to Provide for Protection of Natural Values on Adjacent Protected Lands

60 pages

Microorganisms Found in Field Specimens Of Diseased Corn Borer Larvae

Two hundred and eighty-six abnormal, field collected larvae of the European corn borer were examined for the presence of microorganisms which could have caused the abnormality or death. The most common microorganism found was a microsporidian, Perezia pyraustae Paillot. Next most numerous was a group of enterobacteria. Spore forming rods apparently of the genus Bacillus, and fungi belonging to the genera Beauvaria and Metarrhizium were also isolated. An examination of 100 apparently normal pupae revealed that 82 were hosts to Perezia pyraustae and two contained bacteria

Some Characteristics of Bacteria Isolated From Diseased Larvae of the European Corn Borer

A large proportion of the bacteria isolated from diseased corn borer larvae are pleomorphic, with rods, diplococci, filaments and various transition forms occurring in many cultures. Gram reaction is negative, although the coccoid forms show a tendency to retain gram positivity. In morphology and biochemical characteristics, these isolates resemble certain entomogenous bacteria described by earlier workers, but are similar also to a group of bacteria from human sources which have been designated as members of the tribe Mimeae DeBord. Further studies have been initiated to determine the pathogenicity and the proper taxonomic position of these cultures

Sub-Antarctic and High Antarctic Notothenioid Fishes: Ecology and Adaptational Biology Revealed by the ICEFISH 2004 Cruise of RVIB Nathaniel B. Palmer

The goal of the ICEFISH 2004 cruise, which was conducted on board RVIB Nathaniel B. Palmer and traversed the transitional zones linking the South Atlantic to the Southern Ocean, was to compare the evolution, ecology, adaptational biology, community structure, and population dynamics of Antarctic notothenioid fishes relative to the cool/temperate notothenioids of the sub-Antarctic. To place this work in a comprehensive ecological context, cruise participants surveyed the benthos and geology of the biogeographic provinces and island shelves on either side of the Antarctic Polar Front (or Antarctic Convergence). Genome-enabled comparison of the responses of cold-living and temperate notothenioids to heat stress confirmed the sensitivity of the former to a warming Southern Ocean. Successful implementation of the international and interdisciplinary ICEFISH research cruise provides a model for future exploration of the sub-Antarctic sectors of the Indian and Pacific Oceans

Proteinase-activated receptor 2 modulates OA-related pain, cartilage and bone pathology

Objective Proteinase-activated receptor 2 (PAR2) deficiency protects against cartilage degradation in experimental osteoarthritis (OA). The wider impact of this pathway upon OA-associated pathologies such as osteophyte formation and pain is unknown. Herein, we investigated early temporal bone and cartilage changes in experimental OA in order to further elucidate the role of PAR2 in OA pathogenesis. Methods OA was induced in wild-type (WT) and PAR2-deficient (PAR2−/−) mice by destabilisation of the medial meniscus (DMM). Inflammation, cartilage degradation and bone changes were monitored using histology and microCT. In gene rescue experiments, PAR2−/− mice were intra-articularly injected with human PAR2 (hPAR2)-expressing adenovirus. Dynamic weight bearing was used as a surrogate of OA-related pain. Results Osteophytes formed within 7 days post-DMM in WT mice but osteosclerosis was only evident from 14 days post induction. Importantly, PAR2 was expressed in the proliferative/hypertrophic chondrocytes present within osteophytes. In PAR2−/− mice, osteophytes developed significantly less frequently but, when present, were smaller and of greater density; no osteosclerosis was observed in these mice up to day 28. The pattern of weight bearing was altered in PAR2−/− mice, suggesting reduced pain perception. The expression of hPAR2 in PAR2−/− mice recapitulated osteophyte formation and cartilage damage similar to that observed in WT mice. However, osteosclerosis was absent, consistent with lack of hPAR2 expression in subchondral bone. Conclusions This study clearly demonstrates PAR2 plays a critical role, via chondrocytes, in osteophyte development and subchondral bone changes, which occur prior to PAR2-mediated cartilage damage. The latter likely occurs independently of OA-related bone changes

Ehrlichia ewingii Infection in White-Tailed Deer (Odocoileus virginianus)

Two closely related zoonotic ehrlichiae, Ehrlichia chaffeensis and E. ewingii, are transmitted by Amblyomma americanum, the lone star tick. Because white-tailed deer (Odocoileus virginianus) are critical hosts for all mobile stages of A. americanum and are important vertebrate reservoirs of E. chaffeensis, we investigated whether deer may be infected with E. ewingii, a cause of granulocytotropic ehrlichiosis in humans and dogs. To test for E. ewingii infection, we used polymerase chain reaction and inoculation of fawns with whole blood from wild deer. Of 110 deer tested from 20 locations in 8 U.S. states, 6 (5.5%) were positive for E. ewingii. In addition, natural E. ewingii infection was confirmed through infection of captive fawns. These findings expand the geographic distribution of E. ewingii, along with risk for human infection, to include areas of Kentucky, Georgia, and South Carolina. These data suggest that white-tailed deer may be an important reservoir for E. ewingii